Maybe this is a silly question but here it goes. I am using a Rotor-Gene 6000 with a commercial kit for Uraplasma qualitative detection. This is actually a multiplex reaction: Green channel is Ureaplasma Parvum, Yellow channel is U.Uraliticum and Orange channel is internal control. In my samples I observe the following. I get a signal (almost straight line) in the raw data Yellow channel, but when analysis is performed as specified by the manufacturer in the quantitation analysis channel no signal is seen or any line/curve. The software names these samples as NEG. I am wondering if this is true negative or if this is a case of one probe in the multiplex failing. Fx 9860g manager plus cracked. ![]() Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications. Q-Rex Software is the. QIAGEN Rotor Gene Q: Software V2.0 Instrument Setup Instructions for RT 2. Open the Rotor-Gene Q Series Software 2.0 on the desktop of the computer that is. Factor V gene using the Rotor-Gene Analyzer 3000/6000/Q (Corbett Research) Cat. Revision A (ENG) May 2015 F Installation Guide Trimble Accubid Time. Is it normal to get a signal in the raw channel but no signal in the analysed data?? The IC works fine (nice curve on orange channel) In the Green channel when a sample is negative I get a signal in the raw data BUT ALSO in the quantitation analysis data, below the threshhold of course. Powerquest partition magic 9 download free music.
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